Quantitative proteomic analyses reveal that energy metabolism and protein biosynthesis reinitiation are responsible for the initiation of bolting induced by high temperature in lettuce (Lactuca sativa L.)

Abstract Background Lettuce ( Lactuca sativa L.), one of the most economically important leaf vegetables, exhibits early bolting under high-temperature conditions. Early leads to loss commodity value and edibility, leading considerable waste resources. However, initiation molecular mechanism underlying induced by high temperature remain largely elusive. Results In order better understand this phenomenon, we defined lettuce starting period, (33 °C) controlled (20 phase proteomics is analyzed, based on iTRAQ-based proteomics, phenotypic measurement, biological validation RT-qPCR. Morphological microscopic observation showed that occurred 8 days after treatment. Fructose accumulated rapidly During bolting, 3305 identified proteins, a total 93 proteins exhibited differential abundances, 38 which were upregulated 55 downregulated. Approximately 38% involved in metabolic pathways clustered mainly energy metabolism protein synthesis. Furthermore, some sugar synthesis differentially expressed also associated with production. Conclusions This report first changes lettuce. Our study suggested ribosomal are pivotal components during bolting. could provide potential regulatory for temperature, have applications manipulation breeding.